Introduction to Precision Pilot-Scale Fermentation

When your biotechnology laboratory or production facility needs to scale up fermentation processes from bench to pilot scale, standard equipment often falls short. Inconsistent temperature, pH, or dissolved oxygen control can compromise culture viability, lead to unreliable scale-up data, and cost you valuable time and resources.

The Stainless Steel Fermenter is engineered to eliminate these variables. As a cornerstone of the Stainless Steel Fermenter series, this system is built for biotechnology laboratories, pharmaceutical facilities, and pilot production plants that require unwavering performance for process scale-up, GMP-compliant fermentation, and pilot-scale production. This Stainless Steel Fermenter provides a reliable, reproducible environment for your most critical bioprocesses, with precise control of temperature, pH, dissolved oxygen, and agitation. We understand that in your line of work, scale-up precision is not just a feature—it is a production necessity.

Technical Specifications

Here is a detailed breakdown of the technical capabilities for the Stainless Steel Fermenter. Our models are designed to meet rigorous GMP and pharmacopoeia standards.

• Model/Part Number: BioMatrix-20L

• Total Volume: 20 L

• Working Volume: 70% maximum

• Vessel Material: SUS 316 tank body, SUS 304 jacket, 316L stainless steel headplate

• Design Pressure: P(inside) = 0.3 MPa, P(jacket) = 0.3 MPa

• Polishing Precision: Ra 0.4 to 0.6

• Diameter to Height Ratio: 1:2.5

• Model/Part Number: BioMatrix-30L

• Total Volume: 30 L

• Working Volume: 70% maximum

• Vessel Material: SUS 316 tank body, SUS 304 jacket, 316L stainless steel headplate

• Design Pressure: P(inside) = 0.3 MPa, P(jacket) = 0.3 MPa

• Polishing Precision: Ra 0.4 to 0.6

• Diameter to Height Ratio: 1:2.5

• Model/Part Number: BioMatrix-50L

• Total Volume: 50 L

• Working Volume: 70% maximum

• Vessel Material: SUS 316 tank body, SUS 304 jacket, 316L stainless steel headplate

• Design Pressure: P(inside) = 0.3 MPa, P(jacket) = 0.3 MPa

• Polishing Precision: Ra 0.4 to 0.6

• Diameter to Height Ratio: 1:2.5

• Sterilization Method: In-situ steam automatic/manual sterilization, sterilization temperature 100°C to 130°C; sterilization can be agitated simultaneously for complete medium sterilization

• Aeration System: One air intake with rotameter, mass flow meter, 2-stage sterilization filter, non-return valve; Ring Sparger and Microsparger with Overlay for simultaneous gas delivery; four-way gas inlet (Air, O2, CO2, N2) with four-way TMFC automatic mass flow control

• Agitation System: Mechanical seal or magnetic coupling at top of fermenter; special fermentation blades, 4x baffles, 1x comb-type foam breaker; speed 50 to 1000 rpm ±1%, stepless speed regulation

• Temperature Control: Heating by thermostatic water tank and circulation pump; control range ambient +5°C to 65°C, accuracy ±0.1°C; display range 0°C to 150°C ±0.1°C

• Airflow Control Range: 0.1 to 2.0 vvm, automatic or manual adjustment with flow meter display

• Antifoam Control: Automatic foam detection, automatic antifoam addition by peristaltic pump

• Feeding Control: Peristaltic pump switch control, automatic feeding and computation

• pH Control: Intelligent PID control with peristaltic pump switch for acid and alkali flow, automatic control and measurement; electrode can be sterilized repeatedly

• DO Control Range: 0% to 150%, ±3%, with correlation to speed, feeding, and airflow; at least 10 stages of fermentation process sequence control

• Controller: BioVita S Biology Process Controller with 12-inch touch screen

• Control System: Siemens S7-1200 series PLC with 12-inch LCD touchscreen, English menu

• Control Modes: Manual, Automatic, Sequential (up to 10 control sections), Remote (PC-based), Correlation (DO via speed/airflow/pressure/feeding; pH via acid/alkali)

• Data Processing: Trend curve and batch report analysis; historical data storage and USB export; Excel-compatible format

• System Protection: Password protection for fermentation settings; power failure recovery; adjustable recording intervals; independent module-based parameter transmission for easy maintenance

• Real-time Monitoring: Displays system status, hardware conditions, and fault identification for pumps, heating, mixing, etc.

• Peristaltic Pumps: Standard with 4 high-precision peristaltic pumps

• Detection Electrodes: Temperature, pH, DO, Antifoam (standard); CO2, OD (optional)

• Compliance: GMP compliant construction

Standard Accessories included:

• Fermenter base unit with stainless steel vessel

• 316L stainless steel headplate

• Mechanical seal or magnetic coupling agitation system

• 12-inch touch screen controller

• BioVita S control system with Siemens S7-1200 PLC

• 4 high-precision peristaltic pumps

• Temperature, pH, DO, and antifoam electrodes

• Rotameter for airflow control

• 2-stage sterilization filter

• Non-return valve

• Ring Sparger and Microsparger

• Operation manual with validation documentation

Items Excluded:

• Mass flow meter for automatic airflow control (optional)

• Four-way gas mixing system (optional)

• CO2 and OD sensors (optional)

• Calibration certificate (available upon request)

• Extended warranty (available for purchase)

• Spare electrodes and consumables

• On-site installation and commissioning (available at additional cost)

Pre-installation requirement:

• A stable, level floor with sufficient load-bearing capacity

• A dedicated power outlet matching the unit's specifications

• Adequate clearance around the unit for access and maintenance

• Steam supply for in-situ sterilization

• Cooling water supply for temperature control

• Compressed air supply for aeration system

• Site layout and utility planning before installation

• Operator training on standard safety procedures for pilot-scale fermenter operation is recommended

Key Benefits: Precision, Efficiency, and Safety for Your Lab

Our Stainless Steel Fermenter is more than just a piece of equipment; it is a solution designed to address the real-world challenges you face daily. We have focused on core features that deliver tangible benefits, enhancing your facility's productivity and the reliability of your scale-up outcomes.

• Precise Parameter Control: The system maintains temperature accuracy of ±0.1°C, agitation speed of ±1%, and DO control of ±3%, with intelligent PID control for pH. This directly solves the common pain point of inconsistent culture conditions during scale-up, giving you confidence in your pilot production data. For more information on our range of precision lab equipment, visit our website at https://os4u.com.bd/.

• GMP-Compliant Construction: The 316L stainless steel vessel with Ra 0.4 to 0.6 polishing precision and in-situ steam sterilization meets GMP requirements for pharmaceutical and biotechnology production. This engineering excellence reflects the manufacturer's commitment to bioprocess standards. You can see their full capabilities at https://www.antechscientific.com/.

• Scalable Design: With total volumes from 20L to 50L and working volumes of 30% to 70%, this system serves as an ideal intermediate step in scale-up trains from laboratory to industrial production.

• Versatile Gas Mixing: The four-way gas inlet (Air, O2, CO2, N2) with four-way TMFC automatic mass flow control provides precise gas mixture delivery for demanding fermentation protocols.

• Comprehensive Data Management: Trend curve and batch report analysis, historical data storage, USB export, and Excel-compatible format make reporting, troubleshooting, and audits easier.

• Robust and Durable Design: The stainless steel construction with design pressure of 0.3 MPa inside and in jacket complies with international regulations and ensures reliable data for industrial production.

• Safety and System Protection: Password protection for fermentation settings, power failure recovery, and real-time fault identification for pumps, heating, and mixing ensure a safe working environment.

Solving the Problems That Slow Lab Work Down

We know the frustrations that come with pilot-scale fermentation. The Stainless Steel Fermenter is built to eliminate them.

• Struggling with inconsistent culture conditions during scale-up? The PID control system maintains precise temperature (±0.1°C), pH, DO (±3%), and agitation (±1%) control, ensuring reproducible outcomes across every run.

• Concerned about GMP compliance for pharmaceutical production? The 316L stainless steel construction, in-situ steam sterilization, and Ra 0.4 to 0.6 polishing precision meet GMP requirements, helping you meet regulatory expectations.

• Tired of time-consuming data collection and reporting? The BioVita S controller with trend curve analysis, batch reports, USB export, and Excel-compatible format streamlines your data workflow.

• Need a smooth transition from lab to production? The system offers scalable data for the transition to pilot plants and industrial production, with working volumes from 30% to 70% of total capacity.

By choosing this Stainless Steel Fermenter, you are investing in a dependable partner for your facility's daily operations, designed to deliver bioprocess precision and GMP compliance where it matters most.

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Warranty & Support

We provide a 1-year manufacturer’s warranty covering parts and labor. Technical support, installation assistance, and operational training are also available. Replacement parts and consumables are available throughout the product’s service life.

Call to Action

Request a Quotation or Technical Datasheet: Contact us for more information, customized configurations, and solutions tailored to your specific requirements.