Technical Specifications

The MBL4000-T-F-LED is a trinocular compound microscope from A.KRÜSS Optronic, configured for both transmitted-light brightfield work and incident-light fluorescence. It carries five infinity-corrected plan-achromatic objectives from 4X to 100X, a 360° rotatable Siedentopf trinocular head with a C-mount camera port, and two separate illumination paths: a 5W LED with Köhler illumination for transmitted light and a 3W LED epi-fluorescence unit with a six-position filter wheel. The specifications below cover the standard unit; cameras, dark-field condenser, and phase-contrast objectives are ordered separately, and the MBL4000-T-F-PH-LED variant adds phase contrast.

Model/Part Number: MBL4000-T-F-LED

Microscope Head

• Type: Siedentopf trinocular head, 30° angled

• Rotation: 360° fully rotatable

• Eye distance: 48–76 mm, adjustable

• Beam splitter position 1: 100% for eyepieces

• Beam splitter position 2: 20% for eyepieces, 80% for third tube (C-mount camera connection)

• Eyepieces: WF10X/22 mm with dioptre compensation

Objectives and Magnification

• Type: plan-achromatic, infinity corrected, 0.17 mm cover glass correction

• Revolving nosepiece: 5-way, backwards facing, parfocal spacing

• 4X: NA 0.1, working distance 12.1 mm

• 10X: NA 0.25, working distance 4.64 mm

• 20X (spring): NA 0.40, working distance 2.41 mm

• 40X (spring): NA 0.65, working distance 0.65 mm

• 100X (spring, oil): NA 1.25, working distance 0.12 mm

• Maximum magnification: up to 1000X with 10X eyepieces

Illumination

• Transmitted light: 5W LED, Köhler illumination

• Field diaphragm and brightness control: included

• Colour filter recess: included

• Fluorescence illumination: 3W LED epi-fluorescence

• Fluorescence filter wheel: 6 positions

• Filters installed: B (470 nm) and G (500–550 nm)

Condenser

• Type: double-lens ABBE condenser, NA 1.25

• Centering: for Köhler illumination

• Aperture diaphragm: included

• Height adjustment: included

• Optional: dark-field condenser

Stage and Stand

• Stage dimensions: 182 x 140 mm

• Stage marker scale: 0.1 mm graduation

• Stage travel: 77 x 52 mm

• Stand: metal, coaxial coarse/fine drive, 25 mm range

• Coarse drive: 42.4 mm per revolution

• Fine drive: 0.2 mm per revolution

• Fine drive accuracy: 2 μm

Physical and Electrical

• IP code: IP20

• Microscope dimensions (W x H x D): 230 x 490 x 530 mm

• Fluorescence unit dimensions (W x H x D): 150 x 80 x 220 mm

• Weight: 15 kg

• Operating voltage (microscope): external mains adaptor 100–240 VAC; microscope 5–12 VDC

• Operating voltage (fluorescence unit): external mains adaptor 100–240 VAC; fluorescence unit 5–12 VDC

• Power consumption: 36 W

• Rated frequency: 50/60 Hz

• Ambient temperature: 0–40 °C

• Ambient humidity: 10–90 %, non-condensing

Standard Accessories Included:

• MBL4000-T-F-LED trinocular microscope

• Fluorescence unit with six-position filter wheel, B and G filters installed

• External mains adaptors for microscope and fluorescence unit

• Operating manual

Items Excluded:

• Microscope cameras (MK12, 12 MP; MKTV8, 8 MP)

• MKTV display

• Dark-field condenser

• Phase-contrast objectives (available on the MBL4000-T-F-PH-LED variant)

• Stage micrometer, slides, cover glasses, and prepared slides

Pre-installation Requirement:
Place the microscope on a rigid, level bench with clearance above for the trinocular head and camera, and enough depth for the stage travel and the fluorescence unit at the rear. Connect both external mains adaptors to grounded supplies within 100–240 VAC. Allow the LED illumination to stabilise before critical imaging, and confirm the fluorescence filter wheel is seated with the intended B or G filter before the first fluorescence capture. If a camera is used, seat it in the C-mount before the first session.

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Why This Microscope Belongs in a Fluorescence-Capable Lab

A brightfield microscope with an added fluorescence lamp often feels like two instruments sharing a stand. The MBL4000-T-F-LED fluorescence microscope was built with the two paths separated from the start: a 5W LED Köhler transmitted path for brightfield, and a 3W LED epi-fluorescence path with a six-position filter wheel. Each runs on its own external supply, and the fluorescence unit sits at the rear of the stand rather than crowding the stage.

The optical train is the same one used across the MBL4000 series: five infinity-corrected plan-achromatic objectives, a 360° rotatable Siedentopf trinocular head, and a C-mount port that takes the MK12 or MKTV8 cameras. The beam splitter switches between 100% eyepiece view and 20/80 eyepiece/camera view, so a fluorescence session can be viewed through the eyepieces and captured to camera in the same run. B and G filters are installed, with four more positions free for additional filter sets.

For buyers sourcing through OS4U, the configuration questions are whether phase contrast is needed (the MBL4000-T-F-PH-LED adds it), whether the MKTV8 camera and display belong in the order, and whether the dark-field condenser extends the brightfield work you need. The KRÜSS MBL4000-T-F-LED page lists the full accessory range.

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Key Benefits

Two illumination paths, one stand

The MBL4000-T-F-LED laboratory microscope carries a 5W LED Köhler transmitted path and a 3W LED epi-fluorescence path with independent supplies. Brightfield and fluorescence work happen on the same bench without adding a separate fluorescence attachment.

Filter wheel with room to grow

The six-position filter wheel ships with B (470 nm) and G (500–550 nm) filters installed. Four positions remain free for additional sets, so the fluorescence range can expand without replacing the unit.

Objectives that cover the full range

Five plan-achromatic infinity-corrected objectives from 4X to 100X, including a 100X oil objective at NA 1.25. The 20X and 40X objectives are spring-loaded to protect the lens during focusing.

Camera-ready trinocular head

The Siedentopf trinocular head rotates 360° and carries a C-mount port. The beam splitter switches between full eyepiece view and 20/80 eyepiece/camera view, so the MK12 or MKTV8 camera captures what you see without removing the eyepieces.

Köhler illumination for consistent brightfield

5W LED with field diaphragm, brightness control, and a recess for colour filters. The ABBE condenser centers for Köhler illumination, giving even lighting across the field.

Stage precision that supports measurement

The mechanical stage measures 182 x 140 mm with 0.1 mm graduations and 77 x 52 mm travel. The coaxial coarse and fine drive runs across a 25 mm range, with 2 μm fine-drive accuracy.

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Solving the Problems That Slow Fluorescence Microscopy Down

Struggling to add fluorescence without a second instrument? The 3W LED epi-fluorescence path is built into the stand, with a six-position filter wheel and B/G filters installed. No separate attachment, no bench rearrangement.

Worried about filter flexibility? Four filter positions remain free after the installed B and G filters. Additional sets can be added without replacing the filter wheel.

Losing time switching between visual inspection and camera capture? The beam splitter switches between 100% eyepiece view and 20/80 eyepiece/camera view. Both modes are available without removing the camera.

Getting uneven brightfield illumination? Köhler illumination with a centered ABBE condenser, field diaphragm, and brightness control keeps the field even across the view.

Needing phase contrast as well? The MBL4000-T-F-PH-LED variant adds 10X, 20X, 40X, and 100X phase-contrast objectives and a phase-contrast setting on the condenser, so fluorescence, brightfield, and phase contrast share one stand.

Concerned about protecting high-magnification objectives? The 20X, 40X, and 100X objectives are spring-loaded. If the stage is raised too far, the objective retracts rather than the lens contacting the slide.

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Warranty & Support

We provide a 1-year manufacturer’s warranty covering parts and labor. Technical support, installation assistance, and operational training are also available. Replacement parts and consumables are available throughout the product’s service life.

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